Process for the preparation in pure form of the protease activating blood clotting factor VII, its proenzyme or a mixture of both proteins by means of ion-exchange chromatography
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Nov 6, 2003
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Aug 6, 1999
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Abstract
A process for the preparation in pure form of the protease activating blood clotting factor VII and/or its proenzyme is described in which these are obtained from biological fluids or those obtained in the case of preparation by genetic engineering a,) by anion- or cation-exchange chromatography at a pH below the isoelectric point of the protein to be isolated or b) by a combination of anion- or cation-exchange chromatography with affinity chromatography and/or fractional precipitation at pHs of between 2.5 and 9.0, preferably between 2.5 and 7.2, the affinity chromatography being carried out using calcium phosphate/hydroxyapatite a hydrophobic matrix, a chelate matrix, a matrix which is coated with an immobilized monoclonal or polyclonal antibody directed against the protein to be isolated, or its F(ab) or F(ab).sub.2 fragments. A pharmaceutical preparation and a reagent are moreover described which contain the said protease and its proenzyme.
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