Multidimensional chromatography with ion exchange solid phase extraction
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United States of America Patent
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Issued Date -
Sep 16, 2004
app pub date -
Mar 11, 2003
filing date -
Mar 11, 2003
priority date (Note) -
Abandoned
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Abstract
A sample is fractioned prior to chromatographic separation by a multidimensional purification routine including sequential first and second solid phase extraction procedures having different first and second extraction dimensions such as ph and ion strength. The method is automated and performed with a software programmable automated liquid handler system. The first extraction routine produces step graded fractions that are extracted into step graded subfractions in the second extraction routine. The subfractions are separated in an HPLC column with the output columns going to a detector such as a mass spectrometer or a ultra violet detector.
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