Kinetic assay
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United States of America Patent
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Issued Date -
Sep 2, 2004
app pub date -
Jan 14, 2004
filing date -
May 14, 2001
priority date (Note) -
Abandoned
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Abstract
A hapten-linker-large group conjugate for use in a rapid assay, wherein the assay is kinetic-based not approaching equilibrium, the hapten-linker-large group conjugate being of the general formula: X--W--Y-Z wherein: X is a hapten; W is an optional thioether or ether group;Y is a linker of 10 or more atoms in length; and Z is a large group of sufficient size to provide steric hindrance with respect to the binding of X to a ligand in the absence of Y. Also provided are processes for the production of the conjugates, assay methods and kits. The assays of the invention utilising conjugates of the invention [(5)-OVA] provide a better sensitivity than the same assays with conjugates with shorter linkers [(2)-OVA and 3-OVA].
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