Detection of salmonella cells by fluorescence polarization
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United States of America Patent
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Issued Date -
Jul 15, 2004
app pub date -
Oct 14, 2003
filing date -
Oct 16, 2002
priority date (Note) -
Abandoned
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Abstract
A homogeneous fluorescence polarization inhibition assay is used to test for Salmonella contamination, e.g., Salmonella cells, in a sample. The assay makes use of a tracer comprising a fluorophore conjugated to an oligosaccharide from a Salmonella cell wall lipopolysaccharide. The sample is added to an anti-Salmonella antibody to form a mixture, and a blank fluorescence polarization measurement is taken. The tracer is then added to the mixture. After incubation, the fluorescence polarization of the mixture is measured and the blank reading is subtracted. The level of Salmonella contamination in the sample may be determined from the fluorescence polarization measured in this way.
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