Method of preparation of oligosaccharides
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United States of America Patent
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Issued Date -
May 20, 2004
app pub date -
Sep 19, 2003
filing date -
Nov 15, 2002
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Abandoned
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Abstract
The invention provides a method of cleaving an O-linked oligosaccharide from a glycoprotein. The method comprises the steps of contacting a composition comprising a glycoprotein, wherein the glycoprotein comprises O-linked oligosaccharides, with a solution comprising a borane-ammonia complex to form a mixture comprising the glycoprotein and the borane-ammonia complex, incubating the mixture for a period of time sufficient to cleave the linked oligosaccharides from the glycoprotein, and forming a mixture comprising oligosaccharide alditol products and deglycosylated protein by-products.
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