Reagent and method for immunoanalysis of elastase 1 and method of detecting pancreatic disease
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United States of America Patent
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Issued Date -
May 13, 2004
app pub date -
Sep 30, 2003
filing date -
Mar 30, 2001
priority date (Note) -
Abandoned
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Abstract
A reagent for an immunological analysis of elastase 1, comprising two kinds of latex particles having different particle sizes wherein the latex particles carry two kinds of monoclonal antibodies having different specificities for elastase 1, is disclosed. Further, a method for an immunological analysis of elastase 1, comprising the steps of: bringing a sample to be analyzed into contact with two kinds of latex particles having different particle sizes which carry two kinds of monoclonal antibodies having different specificities for elastase 1, and analyzing the degree of a latex-particles-agglutination caused by an antigen-antibody reaction, is disclosed. Furthermore, a method for detecting a pancreatic disease wherein elastase 1 is analyzed by the above immunological analysis method is disclosed. According to the present invention, elastase 1 can be conveniently and quickly (for example, for 10 minutes) analyzed, without a special equipment as in the case of RIA.
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