Methods and means for gene silencing
Number of patents in Portfolio can not be more than 2000
United States of America Patent
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Issued Date -
Apr 22, 2004
app pub date -
Sep 30, 2003
filing date -
Aug 17, 2000
priority date (Note) -
Abandoned
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Abstract
Provided are insolated DNA vectors which may be based on Agrobacterium binary vectors and which comprise: (a) a transfer nucleotide sequence comprising (i) a plant active promoter, operably linked to (ii) a recombinant tobacco rattle virus (TRV) nucleic acid which may corresponds to all or part of TRV RNA 1 and which includes: a sequence encoding a TRV trans acting factor, and cis acting elements, which confer on the TRV nucleic acid transcript the ability to replicate in the cytoplasm of a plant cell; and a heterologous nucleotide sequence which is foreign to said virus (which may be a cloning site, or a targeting sequence which is capable of down-regulating expression of a target gene); (b) border sequences which permit the transfer of the transfer nucleotide sequence into a plant cell genome. Preferred vectors include pBTA.DELTA.AMP.DELTA.16K (SEQ ID NO: 3) or pBTA.DELTA.MP (SEQ ID NO: 2). Also provided are related materials and methods of use of such vectors e.g. to produce a cytoplasmically-replicating RNA which can be used to silence target genes in plants.
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