Method for the amplification and optional characterisation of nucleic acids
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
Apr 8, 2004
app pub date -
Aug 8, 2003
filing date -
Nov 3, 2000
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
A method for the amplification of a template nucleic acid comprises simultaneously carrying out the steps of reacting a nucleic acid primer with said template nucleic acid, normal DNA precursor nucleotides, at least one modified DNA precursor nucleotide and a DNA polymerase so as to obtain an extended nucleic acid primer, said nucleic acid primer remaining bound to said template; cleaving the modified base-containing extended nucleic acid primer so as to generate a free 3'-OH terminus that is extendible by said DNA polymerase; and repeating steps i) and ii) on DNA fragments thereby generated. The modified precursor nucleotide may be a substrate for a DNA glycosylase or recognised by a 3'-endonuclease and determines the cleavage of the DNA and the site of the cleavage accordingly. The method has significant advantages over existing technologies in that it is more versatile and more flexible with respect to providing a single high throughput process that can be easily adapted to multiple different formats in the fields of DNA detection, quantitation and characterisation.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully