Method of cloning animals
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United States of America Patent
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Issued Date -
Apr 1, 2004
app pub date -
Oct 22, 2003
filing date -
Dec 5, 2000
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention relates to cloning method of animals. The invention includes cell lines, reconstructed embryos and cloned or transgenic animals. In particular, the invention provides method of cloning animals by combining genome of donor cells at specific stages of the cell cycle without uses of chemical products, with activated enucleated oocyte to thereby obtain reconstructed embryos. The invention further relates to methods of culturing animal cells until confluence in normal conditions avoiding negative genetic mutations induced by chemicals. Also, the invention relates to a method of preparing recipient oocytes before nuclear transfer, and to a culture medium improving the in vitro as well as in vivo development of reconstructed embryos.
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