Method of assaying iysophospholipase d activity
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United States of America Patent
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Issued Date -
Mar 18, 2004
app pub date -
Jun 27, 2003
filing date -
Dec 28, 2000
priority date (Note) -
Abandoned
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Abstract
The present invention provides a lysophospholipid of the formula (I) 1 wherein R.sub.1 and R.sub.2 are different from each other and one of them is a saturated or unsaturated fatty acid residue having 8 to 22 carbon atoms, and the other is a hydrogen atom or an acetyl group, and X is a chromophore, a method of assaying a lysophospholipase D activity using the phospholipid as a substrate, a method of screening a substance that inhibits LPLD activity and a reagent and a reagent kit useful for these methods. By the use of the novel lysophospholipid of the present invention as a substrate, a specific and high sensitivity LPLD activity assay becomes attainable. Moreover, by the use of a safe and conveniently detectable chromophore, a number of samples can be assayed rapidly.
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