System and method for determining the presence of methylated cytosines in polynucleotides
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United States of America Patent
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Issued Date -
Mar 11, 2004
app pub date -
Apr 11, 2003
filing date -
Oct 11, 2000
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Abstract
The present invention relates to a method for determining the presence of a methylated cytosine in a first sample comprising a first nucleotide containing compound. The first sample and a reference NCC are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first NCC and the reference NCC is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference NCCs. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference NCCs.
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