Separating and/or identifying polymorphic nucleic acids using universal bases
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United States of America Patent
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Issued Date -
Jan 22, 2004
app pub date -
May 5, 2003
filing date -
May 3, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention provides methods for analyzing polymorphic nucleic acids using duplex separation and/or identification techniques. In the present methods one of the nucleic acids has a sequence which includes universal bases that correspond in position to one or more of the polymorphic positions of the polymorphic nucleic acid. The nucleic acid including the universal bases can either represent the polymorphic nucleic acid and hybridize with a reference strand, or act as a reference strand and hybridize with the polymorphic nucleic acid directly, to form a duplex. Separation and/or identification of the duplex from other duplexes and materials can then be performed.
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