Methods for blocking nonspecific hybridizations of nucleic acid sequences
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United States of America Patent
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Issued Date -
Jan 15, 2004
app pub date -
Sep 3, 2002
filing date -
Aug 31, 2001
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Abandoned
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Abstract
Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid--modified bicyclic monomeric units with a 2'-O-4'-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray.
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