Common ligand universal enzyme assay and compositions for use therein

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United States of America Patent

APP PUB NO 20030228621A1
SERIAL NO

10189327

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides compositions containing a common ligand linked to a detectable moiety and provides methods for the preparation of such compositions. The present invention also provides methods for screening candidate ligands for binding to a NAD binding receptor, which include contacting a receptor with a candidate ligand and a composition of the invention followed by evaluation of receptor binding. The screening method of the present invention has broad applicability and can be used to screen large numbers of a wide variety of ligands. The present invention further provides methods for detecting the binding activity of a putative receptor, which include combining the putative receptor with a composition of the invention and evaluating the level of detectable moiety. The invention also provides kits useful for detection of receptors having NAD binding activity and for screening of candidate ligands that bind to a NAD binding receptor.

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Patent Owner(s)

Patent OwnerAddress
TRIAD THERAPEUTICS INC9381 JUDICIAL DRIVE SAN DIEGO CA 92121

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bertolaet, Bonnie San Diego, CA 2 2
Hansen, Mark R San Diego, CA 14 124
Qin, Yong Poway, CA 85 1289
Sem, Daniel S New Berlin, WI 33 119
Sergienko, Eduard San Diego, CA 13 254
Yu, Lin San Diego, CA 87 856

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