Method for pH-biased isoelectric trapping separation
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United States of America Patent
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Issued Date -
Dec 11, 2003
app pub date -
Jun 5, 2002
filing date -
Jun 5, 2002
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Abstract
A method for altering the initial composition of an ampholytic component containing sample by isoelectric trapping provides for selecting a first isoelectric buffer having a pI value different from the pI value of a first ampholytic sample component in the sample, contacting the isoelectric buffer with the first ampholytic sample component, and obtaining the first ampholytic sample component in a non-isoelectric state on one side of an isoelectric separation barrier at the end of an isoelectric trapping process.
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