System and method for determining the sizes and quantity of polynucleotides with capillary array electrophoresis
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United States of America Patent
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Issued Date -
Nov 6, 2003
app pub date -
Apr 30, 2003
filing date -
May 1, 2002
priority date (Note) -
Abandoned
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Abstract
An electrophoretic separation system configured to determine a size of each of a plurality of sample polynucleotides includes a plurality of sample separation lanes, such as capillaries. Each separation lane is configured to subject a number plurality of respective sample polynucleotides and a respective internal standard polynucleotide (ISP) to electrophoresis. The system also includes a ladder separation lane for subjecting at least two members of polynucleotide ladder to electrophoresis. A processor of the system is configured to determine migration coordinates of (1) the ISP and sample polynucleotides subjected to electrophoresis within each separation lane and (2) at least two of the PLMs. The processor also transforms the migration coordinates of the sample polynucleotides of each separation lane from a migration dimension of their respective separation lane to a migration dimension of the polynucleotide ladder. The sizes of the sample polynucleotides are determined based on (1) the respective transformed migration coordinates thereof and (2) migration coordinates of at least two PLMs.
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