Method for producing transgenic plants with modified 5-aminolevulinic acid biosynthesis, and method for identifying 5-aminolevulinic acid synthesis effectors
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United States of America Patent
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Issued Date -
Oct 30, 2003
app pub date -
Jun 10, 2003
filing date -
Dec 10, 1997
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Abandoned
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Abstract
The invention relates to a method for producing transgenic plants by means of modified 5-aminolevulinic acid biosynthesis, transgenic plant cells, transgenic plant parts, transgenic seeds, and transgenic reproduction material. The invention is characterised in that one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS), selected from a group of feedback regulated ALAS, animal ALAS and bacterial ALAS, an active fragment thereof or an antisense or complementary sequence thereof, are integrated into the plant genome in a stable form. Effectors of natural 5-aminolevulinic acid biosynthesis in plants are found using a method for determining effector efficacy of a test substance in relation to a GSAAT, by: a) determining the enzymatic activity of the GSAAT in the absence of a test substance b) determining the enzymatic activity of the GSAAT in the presence of a test substance; and c) by comparing the enzymatic activities determined under a) and b).
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