Phenotypic screen of chimeric proteins
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United States of America Patent
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Issued Date -
Oct 16, 2003
app pub date -
Dec 9, 2002
filing date -
Dec 7, 2001
priority date (Note) -
Abandoned
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Abstract
In one aspect, a library of nucleic acids that encode different artificial, chimeric proteins is screened to identify a chimeric protein that alters a phenotypic trait of a cell or organism. The chimeric protein can be identified without a priori knowledge of a particular target gene or pathway. Some chimeric proteins include multiple zinc finger domains and can induce, for example, thermotolerance, solvent-tolerance, altered cellular growth, insulin production, differentiation, and drug resistance.
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