Detection by sliding template amplification
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United States of America Patent
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Issued Date -
Sep 25, 2003
app pub date -
Feb 20, 2003
filing date -
Feb 21, 2002
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Abandoned
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Abstract
Methods and compositions are provided for amplifying tandem repeats, particularly for detecting binding events, using a nucleic acid reagent comprising two features, one having a tandem repeat region and the other having an extendable 3' terminus. When the two oligonucleotides are hybridized in the presence of a DNA polymerase and NTPs for replicating the tandem repeat region, repetitive extension of the extendable 3' terminus along the tandem repeat region is obtained. By having labeled NTPs, the amplification can be detected as indicative of the binding event. Kits are provided for performing the method.
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