Reactive monomers for the oligonucleotide and polynucleotide synthesis , modified oligonucleotides and polynucleotides, and a method for producing the same
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United States of America Patent
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Issued Date -
Sep 11, 2003
app pub date -
Nov 14, 2002
filing date -
Mar 18, 2000
priority date (Note) -
Abandoned
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Abstract
The invention relates to the production of modified oligonucleotides and to their use for conjugation reactions. The invention further relates to reagents and to methods for producing aldehyde-modified oligonucleotides that contain aldehydes that are protected (masked) as acetals. Once said acetals are incorporated into the oligonucleotides the oligonucleotides are converted to aldehydes and are used for conjugation. The conjugation reaction can be carried out with the free oligonucleotide or with the oligonucleotide that is still immobilized on the substrate.
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