Use of a nucleotide sequence for enhancing protein synthesis and expression of proteins
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United States of America Patent
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Issued Date -
Sep 4, 2003
app pub date -
Nov 1, 2002
filing date -
Jan 28, 2000
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Abandoned
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Abstract
The present invention is related to the use of nucleotide sequences substantially similar to the cDNA sequence (SEQ ID NO:2:) obtainable from the leader sequence (SEQ ID NO:1:) of the Cocksfoot mottle virus (CfMV) which is capable of enhancing protein synthesis and expression of proteins, especially in plants such as cereals. Also disclosed is a method for producing potential enhancer elements by selecting 5'UTRs having a capacity of producing hairpin loop structures and preparing substantially similar nucleic acid sequences. In addition a method for enhancing the expression in plants as well as the properties characteristic for the nucleotide sequence which are responsible for the enhanced expression.
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