High throughput generation of human monoclonal antibody against peptide fragments derived from membrane proteins
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United States of America Patent
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Issued Date -
Sep 4, 2003
app pub date -
Feb 8, 2002
filing date -
Feb 8, 2002
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Abandoned
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Abstract
Methods are provided for efficient, high throughput screening of antibody libraries against proteins targets, especially membrane proteins. In particular, methods are provided for screening a fully human antibody library against membrane proteins such as HIV coreceptors in yeast. More particularly, a library of human single chain antibodies is screened against peptide fragments derived from extracellular domains of human CCR5 and high affinity monoclonal antibodies against CCR5 are selected.
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