Proteomic determination of protein nitrotyrosine modifications using mass spectrometry
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United States of America Patent
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Issued Date -
Sep 4, 2003
app pub date -
May 1, 2001
filing date -
May 2, 2000
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Abstract
Compositions and methods are provided for identifying oxidative modifications of proteins by mass spectrometric analysis, including MALDI-TOF MS, of protein and peptide fractions of biological samples to determine specific occurrences of nitrotyrosine at amino acid sequence and proteomic levels. Diagnostic methods for diseases characterized by elevated free radicals and oxidative stress, and screening assays for therapeutic agents useful in treating such diseases, are also disclosed.
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