Porcine adenovirus E1 region
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United States of America Patent
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Issued Date -
Jul 31, 2003
app pub date -
Sep 24, 2001
filing date -
Sep 24, 2001
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Abstract
The present invention relates to the characterization of the porcine adenovirus E1 region. The complete nucleotide sequence of the genome of porcine adenovirus type 3 (PAV-3), providing the characterization of the PAV3 E1 region, is described herein. Methods for construction of infectious PAV genomes by homologous recombination in procaryotic cells are provided. Recombinant PAV viruses are obtained by transfection of mammalian cells with recombinant PAV genomes. The PAV-3 genome can be used as a vector for the expression of heterologous nucleotide sequences, for example, for the preparation and administration of subunit vaccines to swine or other mammals.
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