Method for isolating plasmids from suspended bacterial or yeast cells
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United States of America Patent
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Issued Date -
Jul 17, 2003
app pub date -
Dec 4, 2002
filing date -
Mar 21, 2001
priority date (Note) -
Abandoned
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Abstract
The invention relates to a method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and pre-damaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to said suspension to alter the conformation of the plasmids to be isolated in such manner as to promote retention of the cell components in or at the filter matrix, the suspension thereupon being moved through a filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the said filter matrix.
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