Cell proliferation ability evaluation method and apparatus
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United States of America Patent
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Issued Date -
Jul 17, 2003
app pub date -
Dec 26, 2002
filing date -
Nov 22, 2000
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Abandoned
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Abstract
A method for evaluating the proliferation ability of an entire cell population by observing each anchorage-dependent cell without invading and destroying the cell. The evaluation method includes monolayer-culturing anchorage-dependent cells in a culturing chamber, imaging each cell, calculating an index related to the proliferation ability of each cell using the image of each cell, and evaluating the proliferation ability of the cell population using the index. The index includes an expansion speed (rs) indicative of a change in a projected area of each cell (Sa) during a cell adhesion phase, the number of cells contacting each cell in a cell proliferation phase, and a projected area (Sa') of each cell in the cell-proliferation phase.
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