Novel 'oxy' CMS brassica napus corrrected for chlorosis using hexaploid bridging material generated through protoplast fusion and a method of producing the plant
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Jul 26, 2002
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Jan 28, 2000
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Abstract
The present invention relates to a novel cytoplasmic male sterile (CMS) Brassica napus plant containing improved `oxy` cytoplasm i.e. chloroplast from Brassica oleracea and recombinant mitochondria from Brassica oxyrrhina, a process for developing the said `oxy` CMS Brassica napus plants by using hexaploid `oxy` CMS Brassica plants, obtained through somatic hybridization, as the bridging material and backcrossing these for at least five generations to transfer the novel `oxy` CMS character to Brassica napus plants and a method for producing the improved hexaploid male sterile `oxy` CMS Brassica plants mediated through protoplast fusion and regeneration of the somatic hybrids and to the transfer of this improved `oxy` cytoplasm to tetraploid Brassica napus (AACC) plants through conventional backcross breeding for five generations.
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