Methods and compositions for the detection of bacterial endotoxins
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United States of America Patent
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Issued Date -
Jun 5, 2003
app pub date -
Apr 26, 2002
filing date -
Oct 9, 1997
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Abandoned
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Abstract
The invention provides methods and compositions for the detection and/or quantification of bacterial endotoxins. In particular, provided herein is an inexpensive and reproducible method for producing an improved amebocyte lysate preparation having reduced Factor G activity. Provided also is an endotoxin-specific amebocyte lysate preparation produced by such a method. In addition, the invention provides methods and compositions for enhancing the sensitivity to endotoxins of amebocyte lysate preparations having reducing Factor G activity. In particular, the sensitivity of such amebocyte lysate preparations to endotoxins can be enhanced by the addition of exogenous (1.fwdarw.3) .beta.-D-glucan.
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