Methods for detecting a plurality of analytes by chromatography
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United States of America Patent
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Issued Date -
May 15, 2003
app pub date -
Nov 9, 2001
filing date -
Nov 9, 2001
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Abandoned
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Abstract
The invention provides a method for detecting a target nucleic acid sequence. The method involves contacting one or more target nucleic acid sequences with a set of tagged probes under conditions sufficient for hybridization of a target nucleic acid sequence with a tagged probe, the tagged probes comprising a mobility modifier attached to a nucleic acid target binding moiety by a bond that is cleavable by a nuclease, the nucleic acid target binding moiety containing at least one bond resistant to said nuclease; treating the tagged probe hybridized to the target nucleic acid with a nuclease under conditions sufficient for cleavage of the nuclease-cleavable bond to release a tag reporter; separating a tag reporter using a chromatographic method, and detecting a tag reporter corresponding to a known target sequence.
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