Funtional assay for genes involved in xylogenesis
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United States of America Patent
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Issued Date -
May 15, 2003
app pub date -
Nov 8, 2002
filing date -
Nov 9, 2001
priority date (Note) -
Abandoned
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Abstract
The present application relates to a functional assay for high-throughput screening and identification of plant genes involved in xylogenesis and secondary cell wall formation. The assay involves transforming Zinnia mesophyll cells in vitro under non-inducing or transdifferentiation-induc-ing conditions with DNA constructs comprising one or more polynucleotide sequences toa be tested and comparing the expression of the sequences in the non-induced and transdifferentiating cells. The assay is particularly useful for identifying promoters that are active in xylem and xylem-forming tissues and their transcriptional regulators.
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