Process for the production of biologically active polymeric nanoparticle-nucleic acid conjugates
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United States of America Patent
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app pub date -
Jun 19, 2002
filing date -
Oct 23, 1996
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Abandoned
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Abstract
A process is disclosed for producing biologically active polymer nanoparticle-nucleic acid conjugates by polymerizing vinyl monomer with a low-water solubility in an aqueous solution, then reacting the resulting polymer suspensions with the nucleic acids. The process is characterized in that the vinyl monomers are emulsion polymerized in the presence of cationic radical starters but in the absence of any emulsifier. The thus obtained polymer nanoparticle-nucleic acid conjugates are sufficiently stable in biological media and are characterized by a high transport efficiency through cellular membranes.
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