Methods for attaching nucleic acid molecules to electrically conductive surfaces
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United States of America Patent
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Issued Date -
Feb 27, 2003
app pub date -
May 30, 2002
filing date -
Aug 8, 2001
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Abandoned
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Abstract
The present invention relates to a method of attaching nucleic acid molecules to two different electrical conductors, where a first set of oligonucleotide probes is attached to the first electrical conductors with an attachment chemistry which binds the first set of oligonucleotide probes to the first electrical conductors but not to the second electrical conductors. Then, a second set of oligonucleotide probes is attached to the second electrical conductors. The present invention also provides methods for attaching nucleic acid molecules to electrical conductors using a masking agent and methods for attaching nucleic acid molecules to electrical conductors by electrostatic attraction so that the oligonucleotide probes are chemically bound to the electrical conductors. The present invention also discloses methods and devices for detecting a target nucleic acid molecule in a sample.
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