Methods for base counting
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United States of America Patent
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Issued Date -
Feb 27, 2003
app pub date -
Aug 14, 2001
filing date -
Aug 14, 2001
priority date (Note) -
Abandoned
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Abstract
Methods are provided for determining polynucleotide sequence information using mass-modified bases incorporated into amplification products. A sample including a target nucleic acid is amplified in the presence of a mass-modified nucleobase to produce an amplified product incorporating the mass-modified nucleobase. The mass of one strand of the amplified product is compared with the mass of one strand of a reference nucleic acid.
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