Modified microporous membrane for non-specific and sequence-specific nucleic acid capture and methods of use
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United States of America Patent
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Jun 4, 2002
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Jun 4, 2001
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Abstract
Disclosed herein is a novel multi-layered, composite microporous membrane comprising in at least one layer a highly electropositive hydrophilic material distributed throughout wherein the material is capable of irreversibly binding nucleic acid and, optionally, at least one layer where the material is associated with sequence-specific peptide nucleic acids, permitting the simultaneous or sequential capture, amplification and/or identification of specific nucleic acid sequences of interest. Also disclosed herein are methods of use of the composite membranes of the invention in applications based on the sequence-specific capture and/or amplification and identification of nucleic acid from complex biological samples.
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