Cell-based assays for the simultaneous and discrete analysis of multiple analytes
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United States of America Patent
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Issued Date -
Dec 19, 2002
app pub date -
Mar 22, 2002
filing date -
Mar 23, 2001
priority date (Note) -
Abandoned
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Abstract
Multiplexed immunoassays are performed using cells expressing on their surface capture agents such as antibodies or antibody fragments. The cells serve as the solid phase supporting the capture agent and also express identifiers encoding the nature of the capture agent, allowing the cells to be used in multiplexed assays. For example, the identifiers can be internally expressed fluorescent proteins or externally expressed proteins that bind to tagged antibody reagents. Analyte detection and quantification are performed by detection antibodies binding to bound analyte or by detection proteins expressed by the cell in response to analyte binding. By encoding capture, identification, and analyte detection functionalities within the cell, expensive and time-consuming steps of antibody preparation, purification, and coupling to a solid phase are eliminated, making the cells advantageous over antibody-coupled beads currently used in multiplexed immunoassays.
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