Method of agrobacterium mediated plant transformation through treatment of germinating seeds
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United States of America Patent
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Issued Date -
Dec 5, 2002
app pub date -
Feb 19, 2002
filing date -
Feb 26, 2001
priority date (Note) -
Abandoned
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Abstract
The invention relates to a plant transformation method through treating germinating plant seeds with an Agrobacterium strain carrying a foreign DNA fragment . In particular, germinating plant seeds were used as receptors, Ti (tumor inducing) plasmid harbored in an Agrobacterium tumefaciens strain carrying an inserted foreign DNA fragment was used as a gene donor. The germinating plant seeds were co-cultured with the Agrobacterium strain, during the process the foreign DNA fragment was transferred into the donor plant genome. PCR amplification and PCR-Southern hybridization verified that the foreign gene fragment has been transferred into the donor plant and can inherit to the next generation.
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