Multiple simultaneous antigen detection by immunohistochemistry
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United States of America Patent
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Issued Date -
Nov 21, 2002
app pub date -
Sep 6, 2001
filing date -
Apr 27, 2001
priority date (Note) -
Abandoned
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Abstract
A method of simultaneously identifying two different markers in a sample by contacting a sample suspected of containing the two different markers with an antibody solution having a first antibody or fragment thereof against a first marker and a second antibody or fragment thereof against a second marker. The first antibody is conjugated to a first enzyme and the second antibody is conjugated to a second enzyme. The first enzyme and the second enzyme are different. The sample is incubated with the antibody solution for a sufficient time to allow the first marker to bind with the first conjugated antibody and the second marker to bind with the second conjugated antibody. The sample is assayed for a change in enzymatic activity and the presence of the first marker and/or the second marker is determined. The present invention also includes a kit for the simultaneous detection of multiple target molecules.
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