Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules
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United States of America Patent
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app pub date -
Mar 15, 2001
filing date -
Feb 6, 1998
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Abstract
Method and apparatus for high-sensitivity fluorescence detection wherein (I) a sample comprising fluorescent molecules is made to flow through a channel structure (1) including a constricted region (2) with a dimension corresponding to the size of a tightly focused laser spot and with extremely thin, transparent walls; (II) a laser beam (3) is focused inside the constricted (2) and thus exciting molecules passing through the constricted region (2); and (III) the fluorescence emitted due to excitation is detected. This enables direct determination of the concentration of a sample without use of internal or external standards. A method for the production of a flow cell for use in the method or apparatus, wherein a part of a channel structure (1) is heated until its melting point is reached, followed by pulling of the structure to lengthen the melted region and make it thinner until it has a dimension corresponding to the size of a tightly focused laser spot at the diffraction limit.
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