Microbiological production method for alpha-L-aspartyl-L-phenylalanine
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United States of America Patent
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Issued Date -
May 30, 2002
app pub date -
Oct 1, 2001
filing date -
Mar 29, 1999
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a new, microbiological, method for the production of .alpha.-L-aspartyl-L-phenylalanine (Asp-Phe) from the substrates L-aspartic acid (L-Asp) and L-phenylalanine (L-Phe) wherein the substrates are contacted, in the presence of ATP, with a non-ribosomal dipeptide synthetase comprising two minimal modules connected by one condensation domain wherein the N- resp. C-terminal modules are recognizing L-Asp and L-Phe, respectively, and the latter module is covalently bound at its N-terminal end to the condensation domain, and wherein each of these minimal modules is composed of an adenylation domain and a 4'-phosphopantetheinyl cofactor containing thiolation domain, and that the Asp-Phe formed is recovered. The present invention also relates to novel DNA fragments or combination of DNA fragments encoding a new Asp-Phe dipeptide synthetase, micro-organisms containing such DNA fragments, as well as to the new Asp-Phe dipeptide synthetases itself.
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