METHOD OF NUCLEIC ACID SEQUENCING
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United States of America Patent
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Issued Date -
May 9, 2002
app pub date -
Sep 30, 1999
filing date -
Apr 1, 1997
priority date (Note) -
Abandoned
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Abstract
Different nucleic acid molecules present at different locations can be sequenced in parallel Primers that are annealed to the nucleic acid molecules can be provided. Each location can then be provided with a nucleic acid polymerase and a nucleotide. It can then be determined whether or not the nucleotide has been used in primer extension and the process can be repeated. As an alternative to using primers, a nick in a double stranded nucleic acid molecule can provide a 3'-OH group for chain extension.
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