Anti-fungal pharmaceutical compositions comprising an active ingredient prepared from Zingiber officinal
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United States of America Patent
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app pub date -
Jun 22, 2001
filing date -
Aug 26, 2000
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Abstract
A method of preparing an extract which is potent in anti-fungal activity from Zingiber officinale, includes the following steps: preparing a crude liquid from rhizomes of ginger by extraction with an organic solvent or supercritical CO.sub.2, or by distillation with steam; introducing the crude liquid to a reverse phase chromatography column, and eluting the column with water, a first eluent and a second eluent having a polarity weaker than that of the first eluent but stronger than that of chloroform, so that a first eluate resulting from elution of the first eluent and a second eluate resulting from elution of the second eluent are obtained; removing the first eluent and the second eluent from the first eluate and the second eluate by evaporation, respectively, so that a first concentrated eluate and a second concentrated eluate are obtained as the potent extract.
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