Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
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United States of America Patent
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Issued Date -
Nov 29, 2001
app pub date -
Mar 29, 2001
filing date -
Mar 31, 2000
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Abandoned
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Abstract
Disclosed herein is a method for significantly enhancing the luciferase activity in a chemiluminescence assay technique. The disclosed method involves the use of trehalose, a-D-glucopyranosyl-a-D-glucopyranoside, in a chemiluminescent reactant system. By adding trehalose up to its saturation solubility level, increases in emission intensity of from 25-100%, or greater, can be attained.
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