Systems and methods for the detection and analysis of free thiol

Number of patents in Portfolio can not be more than 2000

United States of America

PATENT NO 12265031
APP PUB NO 20240344982A1
SERIAL NO

18604002

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Abstract

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Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms. In some instances, the method further includes applying a reducing agent and quantifying total-thiol content in the sample.

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Patent Owner(s)

Patent OwnerAddress
PROTEINSIMPLE3001 ORCHARD PKWY SAN JOSE CA 95134

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Heger, Christopher Campbell, US 7 6
McElroy, James San Jose, US 27 670

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