Artificial non-coding RNA module for enhancing nitrogen fixation ability of microorganisms

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PATENT NO 12258562
APP PUB NO 20230227827A1
SERIAL NO

18008674

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An artificial non-coding RNA (ncRNA) module constructed by a synthetic biology technique and the use of the artificial ncRNA module in the construction of an artificial nitrogen fixation system are disclosed. The RNA module can enhance the post-transcriptional stability of nifHDK mRNA by interacting with a nitrogenase coding gene nifHDK mRNA, thereby improving the nitrogen fixation ability of a chassis microorganism. A fusion expression vector carrying the artificial RNA module is constructed and transformed into different chassis nitrogen-fixing microorganisms. It is confirmed through experiments that, under nitrogen fixation conditions, the artificial RNA module of the present disclosure can significantly improve the nitrogenase activity of a recombinant engineering bacterial strain.

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Patent OwnerAddress
ZHONGNONG CHUANGDA (BEIJING) ENVIRONMENTAL PROTECTION TECHNOLOGY LTDZHONGGUANCUN SOUTH NO 12 HAIDIAN DISTRICT BEIJING 101405 CN BEIJING 101405

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Inventor Name Address # of filed Patents Total Citations
Ke, Xiubin Beijing, CN 2 0
Lin, Min Beijing, CN 83 503
Yan, Yongliang Beijing, CN 3 0
Zhan, Yuhua Beijing, CN 2 0

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