Membrane protein activity measurement method
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United States of America
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Mar 11, 2025
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Oct 7, 2021
app pub date -
Jul 26, 2019
filing date -
Jul 26, 2019
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Abstract
The present invention provides a comprehensive assay capable of comprehensively measuring interactive factors for a variety of different types of proteins. In the present invention, provided is a system for detecting activation of a protein by integrating, with mediation of a plurality of factors, changes in the transcriptional regulatory region of a gene whose expression is changed by activation of the protein. The stimulation response ratio of transcription activity before and after stimulation by ligands possessed by individual transcriptional regulatory regions is synergistically integrated, so that a highly sensitive reporter assay system can be constructed.
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