Importance

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Abstract

A system and method for solid phase chemical synthesis reduces the overall amount of solvent required for the process. In, for example, solid phase peptide synthesis, during deprotection of the bound peptide, a deprotecting agent, such as piperidine in dimethylformamide (DMF) is introduced to the reaction vessel. After deprotection is complete, pure DMF, or other similar wash solvent, is used to wash away the residual piperidine from the reaction vessel. The used wash solvent can be removed from the reaction vessel, warmed to evaporate off the piperidine, and the purified used wash solvent can be cooled and re-introduced into the reaction vessel for a subsequent washing. Thus, instead of using six volumes of DMF for washing six times, for example, a single volume of DMF can be used, purified and reused, thus substantially reducing the volume of DMF needed for washing the deprotecting agent from the reaction vessel.

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First Claim