Systems and methods for chromatography use and regeneration
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United States of America
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Mar 11, 2025
Grant Date -
Oct 13, 2022
app pub date -
Sep 23, 2020
filing date -
Sep 23, 2020
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Abstract
Aspects of the present disclosure relate to a method of regenerating a hydrophobic interaction chromatography column to which a load mass has been applied, the method comprising passing one or more column volumes of an alkaline solution through hydrophobic interaction media within the column, wherein the alkaline solution exhibits a pH of between about 10 and about 14, and a conductivity of between 0.5 mS/cm and about 10 mS/cm, wherein material bound to the hydrophobic interaction media is removed. In some cases, the alkaline solution may include sodium hydroxide at a concentration of between, e.g., about 0.1 mM and 10 mM.
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