STED microscopy method with improved signal to noise ratio in low photon count imaging conditions
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United States of America
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Mar 4, 2025
Grant Date -
Oct 27, 2022
app pub date -
Oct 8, 2020
filing date -
Oct 8, 2020
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Abstract
The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.
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