Method for gas enrichment and simultaneously for displacement of a fluid, and system for controlling the cell environment on a corresponding multi- well cell culture plate
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United States of America
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Mar 4, 2025
Grant Date -
Jun 8, 2023
app pub date -
Jan 31, 2023
filing date -
Jan 31, 2023
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Abstract
A method for displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.
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