Detection method using kit for detecting plurality of target nucleic acids

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United States of America

PATENT NO 12234508
APP PUB NO 20220042071A1
SERIAL NO

17276654

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Abstract

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The present invention provides a method for detecting multiple target nucleic acids, the method making it possible to amplify a plurality of genes using one reaction vessel containing therein one type of reaction solution and further using a single label. Computation is performed in accordance with the following formulas for every specific cycle and/or every cycle during amplification reaction using a kit for detecting the multiple target nucleic acids. (Formula 1): f1[n]=fhyb.1[n]/fden.1[n], (Formula 1′): f2[n]=fhyb.2[n]/fden.2[n], (Formula 2): Fr[n]=(a−f2 [n])/(a−f1 [n]).fhyb.1[n]: Fluorescence intensity value in elongation step of first target nucleic acid detecting step. The same hereinafter.

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Patent Owner(s)

  • MIZUHO MEDY CO., LTD.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ebisu, Koichi Saga, JP 1 0
Ichimaru, Kazuhiro Saga, JP 3 33
Nagano, Takashi Saga, JP 84 1061
Narahara, Kenji Saga, JP 8 19

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